A Note on Efficient Isolation of Genomic DNA from Acacia catechu Willd

Abstract

An efficient and convenient method of extraction of the genomic DNA of high quality and good amount from Acacia catechu Willd., required for the molecular characterization and genetic divergence studies is presented here. Various problems are encountered due to the presence of polysaccharides, polyphenols and secondary metabolites. The presence of high content of secondary metabolites and phenolics interfere with the extraction of genomic DNA and hinders in carrying out the polymerase chain reaction. Also the yield of DNA obtained is greatly affected. An attempt has been made to extract DNA by using a modified CTAB method which results in good yield and good quality of DNA .