Influence of BAP× GA3 on indirect shoot regeneration fromthe two different explants of Carthamus tinctorius L. Var PKV-Pink.

Abstract

Safflowercommonly known as ‘Kusum’ is an important oilseed crop cultivated in several parts of India and it is widely exploited for its medicinal properties. An efficient protocol for in vitro shoot regeneration from two different explants of Carthamus tinctorius L. Var. PKV-Pink. Hypocotyls and cotyledonary leaves were used inthe present investigation and those were obtained by invitro germinating seedlings of 10-daysold.Both explant cultures were raised onMurashige and Skoog’smedium supplementedwith different concentrations of either BAP(1.0-6.0 mg/L) or in combination with NAA (0.5 mg/l).Maximumpercentage of shoot initiation (76.65%) in hypocotyls explant was observed on a medium fortified with BAP (5.0mg/L). While in cotyledonary leaves, it was observed on a medium fortified with BAP (4.0mg/L). Subsequent culturing of these responded explants resulted into maximum percentage of shoot multiplicationon a medium fortified with BAP (5.0mg/L) + GA3 (1mg/L) for hypocotyls and BAP (4.0mg/L) + GA3 (1mg/L) for cotyledonary leaves. Proliferated and well developed shoots were then rooted in medium having different concentrationsof NAA. Completerooting was observed within 47-50 days of culture. The rooted plantlets were subjected to an acclimatization process.