In-Vitro Callus Induction From Two Different Explants Cotyledonary Leaves And Hypocotyl In carthamus- tinctorius linn. Var pkv-pink.

Abstract

Callus from Safflower (cv. PKV-Pink.) was raised from two different explants hypocotyls and cotyledonary leaves. The explants were cultured from the one to two weeks old seedlings and transferred to an MS medium supplemented with kinetin and 2, 4-D with different concentration. The highest percentage of callus induction (99.99%) was observed in MS with equal concentration of 2, 4-D and KIN (1 mg/l each) in cotyledonary nodes as compare to hypocotyls and also an attempt are made to standardized the seed surface sterilization protocol by using different sterilizing agents.