Molecular characterization of inbreds in maize (Zea mays L.)

Abstract

A set of 8 inbred lines viz., HKI-163, HKI-1105, HKI-1128, DMRPE6, CML-285, CML-292, CML-132, LM-16 were analyzed using 36 Simple Sequence Repeats (SSR) markers 22 markers out of 36 were found to be polymorphic. In total 50 SSR alleles were identified with a mean of 2.27 alleles per locus. The average polymorphic information content (PIC) was 0.41. These markers showed high level of polymorphism and therefore can be use for fingerprinting and mapping studies. Genetic similarity expressed as Jaccard’s coefficient varied from 0.57-0.15. Three clusters were obtained by using Unweighted Paired Group Method using Arithmetic Averages (UPGMA). Coefficient of similarity matrix showed inbred lines CML 292 and CML285 tobe more similar while CML132 and DMRPE6 were found to be most diverse. Inbred lines used in present study revealed heterozygosity ranges from 4.3-17.4%. In this study we found SSR as a good tool for characterization of maize genotypes.