Gynogenic approach of haploid induction in eggplant (Solanum melongena L.)

Abstract

To determine the potential for haploid induction via in vitro gynogenesis in eggplant, the ovaries from five different eggplant cultivars were selected. Un-pollinated ovaries were cultured on six selected induction media to induce in vitro gynogenesis. During the culture, ovaries enlarged markedly, with callus having opaque white color appearance which after transfer to regeneration media showed subsequent greening. Continuous sub-culturing in the regeneration medium MS + Zeatin 1.0 mg L-1 + Kinetin 3.0 mg L-1 led to leaf primordia development and shoot regeneration in the cultivar HD-2. The results show that stage of flower bud and growth hormone combinations play an important role in callus induction as well as regeneration. Gynogenesis can be used as an alternative approach for haploidy induction in the recalcitrant Solanaceous crops.