Tannase from Novel Bacterial Isolates – Paved the Way of Industries

Abstract

Bacteria capable of tolerate high levels of hydrolyzable and condensed tannins by producing extracellular tannase were isolated from different sources viz., tea garden soil, pine forest soil, ruminial fluid and sheep excreta. Based on morphological and biochemical identification using ABIS software these were identified as Klebsiella sp, Enterobacter sp, Staphylococcus sp and E. coli. Secondary screening revealed that genus belonging to Klebsiella and Enterobacter were the best enzyme producer (0.38Uml-1 and 0.30Uml-1) and thus were identified using molecular methods. Nearly complete sequences of the small-subunit rRNA genes, which were obtained by PCR amplification and sequencing, were used for phylogenetic characterization which showed concordance results. The phylogenetic analysis based on the comparisons of the 16S rRNA gene sequences showed that the isolate Klebsiella was mostly related to Kleibsiella variicola KT261225 (with the sequence similarity of 98.33%) and isolate Enterobacter with Enterobacter hormaechei KT215540 (95.48%). This work paved the way for isolation of various tannin tolerant bacteria which is not restricted by climate, geography, or host animalis, although number of microbes vary according to tannin content of a particular area.