Polymorphism of FecB Gene in Black BengalGoat by PCR-SSCPmethod

Abstract

The objective of present study was to search for FecB gene polymorphism in Black Bengal goats. Blood samples were collected from 96 animals. Genomic DNA was isolated using phenol-chloroform extraction method. The quantity and quality of isolated DNA was checked by agarose gel electrophoresis and spectrophotometery. W ith the primers having forward and reverse base sequence as 5'-CCAGAGGACAATAGCAAAGCAAA-3' and 5'- CAAGATGTTTTCATGCCTCATCAACAGGTC-3' respectively, PCR products of 190 bp were obtained. For genotyping, amplified products were subjected to polyacrylamide gel electrophoresis for the detection of Single Strand Conformational Polymorphism (SSCP). As a result, three different SSCP variants were found which were designated as AA, AB and BB. The highest genotype frequency was observed for AB (0.38), which was followed by BB (0.33) and AA (0.29). Nucleotide sequences of the allelic variants were also analyzed. The DNA sequences showing polymorphism observed were used to identify SNPs. Principal SNP was found at 78 position of gene sequence, which shows transition of Adenine (AA genotype) to Guanine (BB genotype).