Genetic fingerprinting of different cultivars of wheat by using RAPD marker

Abstract

Genetic diversity analysis of nine wheat (Triticum astivum) genotypes was carried out using Random Amplified Polymorphic DNA (RAPD) markers. The 15 RAPD primers amplified a total of 109 bands, out of which 73 were polymorphic. The size of amplified DNA fragment varied from 938.46 to 988.88 bp. The polymorphic bands varied from 45percent in OPA-09 to 100 percent in OPA-12. Dendrogram based Jaccard’s similarity coefficient grouped allnine genotypes except one i.e PBW343into one single cluster encompassing threesubclusters. The first subcluster comprised two genotypes viz; NIAW1415 and NIAW301while second subcluster grouped another four genotypes viz; MACS6222, DBW17, NI5439 and NIAW1885. The third subcluster was comprised remaining two genotypes viz; NIAW2030 and NIAW917. The similarity index values ranged from 0.22 to 0.92 indicating the presence of enormous genetic diversity at molecular level. Therefore, RAPD analysis could be used as tool for detecting genetic diversity and can be precisely used for grouping and selection of diverse wheat genotypes.