Molecular characterization in Fieldpea(PisumsativumL.vararvense)

Abstract

DNA-based markers provide powerful and reliable tools for discriminating variations within crop germplasm and for studying evolutionary relationships. The present study involved molecular characterization of 36 genotypes of Fieldpea(PisumsativumL.vararvense) comprising 8 parent and 28 F1’s hybrids, using RAPD markers.Pooled RAPD analysis produced a total of 108 DNA fragments, out of which 91 were polymorphic (82.35%) average polymorphism. Parent IPFD 10-13 and KPMR 400 were least similar to each other, the cross between ADARSH X NDP-1 and DF-1 X PRAKASHrevealed lowest similarity index indicating more distance between them. A wide range of genetic similarity (0.36 to 0.89) conform that a significant genetic variation exists among the various cross combinations of fieldpea. It can be further utilized in strengthening fieldpea breeding programme for improving yield and quality characters of fieldpea.