Study of genetic diversity in wheat genotypes using microsatelite markers

Abstract

Molecular analysis for advance lines of hexaploid wheat (Triticum aestvium L.) cultivars was investigated by applying microsatellite markers. The plant materials consisted of 30 advance lines of Triticum aestivum obtained from Aldan, HD 29, WH 542 and WH 711. The advanced lines and their parents were classified in 7 clusters on the basis of Tocher’s method with cluster-I containing the maximum of 11 genotypes. Clustering pattern revealed that variable number of advance lines were included into different clusters and also indicated the presence of considerable amount of genetic diversity in this material. Maximum intra-cluster distance (3.95) was observed in cluster-III indicating greater genetic divergence between the genotypes belonging to this cluster. Maximum inter-cluster distance (7.87) was recorded between cluster-V and III followed by cluster-III and VII (7.54) indicating wide genetic diversity and it may be used in wheat hybridization programme for improving grain yield. Seven primers out of 35 SSR primers showed polymorphism in banding pattern. A total of 15 alleles were detected. The number of alleles per locus ranged from 1-3 with an average of 2.14 alleles per locus. The overall size of PCR products amplified ranged from 100-300 bp.