RAPD in goat and application of Shannon’s index and AMOVA

Abstract

This experiment was conducted in the Department of animal breeding and genetics , Ranchi Veterinary college, Birsa Agricultural university, Ranchi. Fifty blood samples of Black Bengal Goat were collected from different villages of Purulia district of West Bengal and another fifty blood samples of unrelated individuals of Black Bengal type goat from Jharkhand were collected from different villages of Ranchi district (This goat population is referred as Jharkhand Black goat in this study). Ten Random primers were used to determine the RAPD polymorphism in the two populations, out of which only eight primers were used as Primer-1 and primer-2 did not produce any significant amplification products. A total of 18 bands were generated from 8 random primers. Data were analyzed by using a computer program POPGENE. Shannon’s Information index was observed to be 0.6792 and 0.5898 for Black Bengal and Jharkhand Black respectively. It was found from AMOVA that the percentage variation component among the population and within population were 1.69983 and 98.30017 respectively. The variation within population was much more marked than between the two populations. Therefore there is ample scope for further improvement in its productivity through appropriate breeding strategies.