The Effects On Growth And Salt Tolerant Of Capsicum Annuum

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Naresh Kumar Verma
Manoj Kumar

Abstract

2, 4-D (2mg/l) is the most excellent supplementation to MS medium for initiation as well as best growth of Capsicum annuum callus. Hypocotyl explant showed best results compared to stem, leaf and cotyledonary leaf in terms of qualitative as well as quantitative growth. Hypocotyl is at the junction of differentiation of root and shoot. Since, root did not exhibit callus induction, hypocotylar region appears to accumulate growth promoting hormones which get stimulated upon external supply of nutrient medium with 2, 4-D leading to early proliferation response. Callus showed least growth in 200mM NaCl added respective sets compare to salt free controls. Both hypocotyls and cotyledonary leaves used as explants responded to increasing concentration of salt with increase in proline and protein content, where as leaf responded through increase in both phenolics and proline content along with protease activity and stem responded to salt in term of proline alone. At 150mM, synthesis of new protein is indicated even under higher protease activity in cotyledonary leaves and leaves. Thus, higher proline upto 150-200mM NaCl is indicating a salt induced osmoregulation response as compatible solute accumulation where as higher protein synthesis even under high protease activity is indicative of specific salt associated defense proteins to have been synthesized in cotyledonary leaves and leaves. As compared to leaves, cotyledonary leaves were not associated with salt induced phenolics synthesis indicating leaves to have more complicated defense system activated against salt stress involving both primary and seccondary levels of metabolism. Hypocotyl was susceptible in term of decline in proline at 150mM NaCl, yet due to higher protein synthesis and poor protease activity, probably it sustained salt stress better and continued callus growth in vitro. In the present case except leaf and stem calli (at lower concentration), RS remained lower than controls. In cotyledonary leaf callus, TS also decreased with salt stress and remained lower than control. In hypocotyl, stem and leaf calli maximum accumulation of TS occurred at 100mM, 150mM and 50mM NaCl respectively. In all types of explants maximum accumulation of ascorbic acid with salt stress occurred in hypocotyl callus suggesting it to be the most salt tolerant part than other types of explants.

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How to Cite
Verma, N., & Kumar, M. (2019). The Effects On Growth And Salt Tolerant Of Capsicum Annuum. Indian Research Journal of Genetics and Biotechnology, 11(02), 85-91. https://doi.org/.
Section
Review Article