Modified Protocol for Plant Genomic DNA Isolation

Abstract

Despite optimism about continued yield improvement based on conventional breeding approaches, a modern technology such as biotechnology is necessary to maximize the probability of success. One area of biotechnology is DNA marker technology, derived from research in molecular genetics and genomics, offers great promise for plant breeding. By using DNA markers to assist in plant breeding, efficiency and precision could be greatly increased. High-resolution genetic mapping of molecular markers for marker assisted selection of targeted trait require high quality of DNA isolation from a large number of plants in short time. However, many protocols have been developed and reported for extraction of DNA with the primary aim of development of a relatively quick, inexpensive and consistent protocol to extract high quality DNA with better yield. The general principle of all these DNA extraction protocols remains the same involving disruption of the cell wall, cell membrane and nuclear membrane to release the highly intact DNA into solution ensuring removal of the contaminating biomolecules such as the proteins, polysaccharides, lipids, phenols and other secondary metabolites by enzymatic or chemical methods.