Evaluation of the Hepatoprotective effect of Pollen and Bee Bread against Staphylococcus aureus Infected balb/c mice

Abstract

Pollen 'the male plant reproductive part' is a rich source of proteins, collected by worker honey bees for the rearing of their larvae. Before feeding pollen to its larvae honey bees add some enzymatic secretions and store it in the cells of the comb as 'bee bread'. It was investigated whether water extract of pollen and bee bread exerts hepatoprotective effect against Staphylococcus aureus infected BALB/c mice. The in vivo liver damage of BALB/c mice weighing between 25 to 30 g was induced by intraperitonial injection of S.aureus (5x106 CFU/ml bacterial solutions). All the animals were sacrificed on the 15th day by decapitation. S. aureus induced toxicity produced oxidative stress in mice which caused increase in Lipid peroxidase (LPO) and decrease in Glutathione (GSH), Chloramphenicol acetyl transferase (CAT), Superoxide dismutase (SOD) and Glutathione S transferase (GST) levels. The treatment with pollen and bee bread during the present study caused decrease in the level of LPO and increase in other antioxidant enzymes which showed the positive effect of both bee products pollen and bee bread against bacteria. The study concluded that the water extract of pollen and bee bread had protective effect against Staphylococcus aureus induced toxicity in mice liver