Effect of different media and genotypes on In Vitro Callus Induction in Fenugreek (Trigonella foenum-graecum L.)

Abstract

The study examines the effect of genotypes and culture media on the induction of fenugreek callus (Trigonella foenum-graecum L.). Shoot apex explants of different genotypes were inoculated into MS medium containing concentrations of cytokinins and auxins individually or in combination. The cultures were incubated at a temperature of 25 ± 2° C and an intensity of 3000-3500 lux. Among all genotypes, the maximum callus induction was found in RMt-303 in a shoot apex explant on MS medium at the responsive level (0.75 mg / L BAP + 0.75 mg / L 2, 4-D). MS medium induced maximal callus in shoot apex explants compared to other media supplemented with 0.75 mg / L BAP + 0.75 mg / L 2,4-D.